Over the past few years, a major health problem has emerged and recognized in the resistance to antimicrobial agents by pathogenic bacteria. In this regard, it is well -known that Silver bio -nanoparticles (AgNPs) have bacteriostatic and bactericidal effects. Therefore, this current research will concentrate on the synthesis of metallic bio -nanoparticles of Silver through the use of a reduction of a queous Ag+ ion, with the culture supernatant of Pseudomonas aeruginosa, which has been identified by 16S-r-RNA and deposited in the Gene Bank under Accession No.: 3NP0614. Thus, the AgNPs were assessed with regard to their antimicrobial activities against the various human pathogenic microorganisms. As for the most effective antimicrobial activity of the AgNPs, it was observed to be against the following: Staphylococcus aureus MAM-1, Proteus sp. MAM-2, Klebsiella sp. MAM-6, and E.coli MAM-5; whereas only mo derate antimicrobial activity was seen against Proteus sp., which the concentration of 0.435 ug/ml was the best one. In addition, filter paper, cotton clothes, PVA polymer and gauze were used as immobilizing materials for the AgNPs. Furthermore, gamma ray has been used as sterilizing agent for immobilized the AgNPs on gauze to be used as wound dressing. 2kGy was the best sterilization dose for gauze, immobilized with the AgNPs by a 25-mm inhibition zone.