Journal of Ecology of Health & Environment
Journal license

Journal

Journal of Ecology of Health & Environment


Volume
& Issue

Volume 6, Issue 1


Published
on

January 1, 2018


Pages

15-24


DOI

Article

Isolation and Identification of Cellulose Degrading Bacteria from Different Sources at Assiut Governorate (Upper Egypt)

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Authors

Mohammed Rawway* Affiliation:
Botany and Microbiology Department, Faculty of Science, AL-Azhar University, Assiut, Egypt
, Salah G. Ali Affiliation:
Botany and Microbiology Department, faculty of Science, Al-Azhar University, 71524 Assiut, EGYPT
and Ahmed S. Badawy Affiliation:
Botany and Microbiology Department, Faculty of Science, AL-Azhar University, Assiut, Egypt.


Abstract

The cellulolytic bacteria were screened out from five different sources on two selective media, cellulose agar and carboxymethyl cellulose agar. Ten bacterial isolates characterized by high potent ability to produce cellulases enzymes. These isolates were characterized and identified. The extracellular cellulases activities ranged from 4.389 to 7.724 U/ml for filter paper cellulase (FPCase) and 6.175 to 12.152 U/ml for carboxymethylcellulase (CMCase) assay. Two isolates (CDB6 & CDB10) were found to be highest productivity of cellulases enzymes, 7.268 and 7.724 U/ml respectively, on FPCase, whereas on CMCase assay maximum activity was 11.077 U/ml by CDB6 and 12.152 U/ml by CDB10. Ten cellulose degrading bacterial isolates were identified as Bacillus subtilis (four isolates: CDB6, CDB8, CDB9 and CDB10), Bacillus thuringiensis (two isolates: CDB1 and CDB5), Brevibacillus brevis (two isolates: CDB2 and CDB4), Brevibacillus parabrevis (one isolate: CDB7) and Bacillus pumilus (one isolate: CDB3).


Keywords

FPCase: Filter Paper Cellulase, CMCase: carboxymethylcellulase, CDB: cellulose degrading bacteria


Citation

Rawway, M., Ali, S. G., & Badawy, A. S. (2018). Isolation and identification of cellulose degrading bacteria from different sources at assiut governorate (upper egypt). Journal of Ecology of Health & Environment, 6(1), 15–24. https://doi.org/10.18576/jehe/060103

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