Chitinases assume a critical part in the disintegration of chitin and result in the usage of chitin as a renewable source. A sum of 27 chitinolytic microorganisms was segregated from the sediment samples. Taking after essential and auxiliary screening in colloidal chitin medium, the most potential strains showed the most elevated chitinolytic activity was genetically identified as Aspergillus flavus MK20. Colloidal chitin at concentration 0.3 gm /100 ml as well as the concentration of yeast extract and the nitrogen source at the same concentration gave 620.5 U/ml. Expansion in chitinase yield was watched when Plackett-Burman and Box-Behnken were applied on the selected isolate. The optimum ingredients consisting of yeast extract, peptone, and di potassium hydrogen phosphate for chitinase generation (1520.41 U/ml) by the isolate were observed to be 3.5 g/l, 2 g/l and 3 g/l separately. The outcomes got in the present work demonstrated that isolated fungi had an intense maker of chitinase and the catalyst can be capitalized for the biodegradation of chitinous end products and may discover applications as biocontrol operators against organisms.