In the present study, monthly and seasonal fluctuations of densities of terrestrial spiders were recorded in six different sites at Qena Governorate during the period of one year (February, 2012 - January, 2013). The study revealed the occurrence of 1247 specimens belonging to 14 families and including 23 species of order Araneae. Family Salticidae recorded the highest number during the whole period of study (278 specimens) while family Agelenidae recorded the lowest number in the same period of study (4 specimens). It was observed that the maximal number was collected from Thanatus albini (199 specimens), while Halodromus barbarae was the least species in number since only 2 specimens were collected. The densities of the recorded spiders varied seasonally and the general seasonal peak was recorded during autumn (363 specimens), while the lowest density was observed during winter (215 specimens). Regarding the sex ratio of the collected spiders from all sites, it was clear that there were 213 adult male specimens, whereas the adult females were represented by 440 specimens. Ecological factors which may influence the distribution of the recorded spiders were also recorded; Air temperature (oC), relative humidity (%), evaporation (mm) and wind velocity (Knots).