An indigenous bacterial isolate MAM -P39 was isolated from petroleum polluted soil by selective enrichment with pyrene as the sole source of carbon and energy. During growth on pyrene, the bacterial cell density was monitored by measuring the O.D600. Extracellular protein and bacterial count were determined. The degradation percentage of pyrene was quantified by high -performance liquid chromatography (HPLC). The isolate MAM-P39, which has been identified by 16S rDNA, was the best pyrene degrader. This isolate was identified as Pseudomonas panipatensis MAM-P39 with accession number MF150314. It could degrade 90.5% and 66.03% of 500 and 2000 μM pyrene. Degradation products of pyrene were identified by gas chromatography-mass spectrometry (GC-MS) analysis.