Sediment samples were collected from the shallow areas of the Suez Gulf during winter 2014. Eight locations were selected for this study included El-Tour, RasSidr, Suez North, Suez Middle, AdabiaHarbour, Ain Sokhna, RasGharib and RasShokheir . The physico - chemical characters of the samples were determined. The highest count of the acteniomycetes recorded in Adabia harbor (22cfu/gm) and the lowest count recorded in Ain sok hna station (6cfu/gm). Out of 41 actinomycetes isolates, only 11 isolates showed antimicrobial activities against the bacterial pathogens Bacillus subtillus6633, Staphylococcus aureus 25923, Pseudomonas aeruginosa 902 , Bacillus cereus , Salmonella typhimur ium 14028, Escherichia coli 19404, and Vibrio damsela . Two isolates identified by molecular techniques as Streptomyces rochei HMM13 and Streptomyces sp., these two isolates were able to produce gold (0.035, 0.013), silver (0.376, 0.355) and zinc ( 4.080) nanoparticles. Plackett -Burman Design were applied for optimizing the production of nanoparticles by Streptomyces rochei HMM13 against S. typhimurium 14028, E-coli 19404 and Vibrio damsela .The productivity increased by 1.16 , 1.13 and 1.32 fold, respectively.