The aim of this study was to investigate the relationship between the spatial skills and anxiety levels of pre-service primary-school teachers. Gender that was considered as an important variable on spatial ability and spatial anxiety was also taken into account in the study. Totally 267 pre-service primary-school teachers studying at the third grades of totally four universities including two in the province and two in the district area participated into the research. “Spatial Visualization Test” and “Spatial Anxiety Scale” were used for collecting the data from pre-service teachers. It was determined as result of the study that pre-service male teachers were more successful rather than the female pre-service teachers in terms of spatial skills. Furthermore, female pre-service teachers were noticed to have higher spatial anxiety rather than the male pre-service teachers. Also it was found a significant negative correlation between the spatial anxiety and spatial skill. Some suggestions were offered within the framework of obtained findings.