Background: Functional dyspepsia is considered one of the most common functional gastrointestinal disorders. However functional dyspepsia is not life threatening, it impairs quality of life, has a significant negative impact on work productivity, and increases health care expenses for both the patients and society. Aim: was to study clinical, and histopathological features of patients with functional dyspepsia according to Rome IV criteria in Sohag University Hospital. Materials and Methods: A cross-sectional study was conducted among One hundred and twenty consecutive adult patients with functional dyspepsia according to Rome IV criteria. in Sohag University Hospital. All patients subjected to complete physical examination, esophagogastroduodenoscopy, with multiple gastric and duodenal biopsies for histopathological assessment. Results: 56.5% of laboratory technicians, nursing staff had good knowledge, 65.02% had a positive attitude and 53.81% had bad practice toward HCV patients. Laboratory technicians have better knowledge, more positive attitudes and better practices than nursing staff. Conclusion: Knowledge, attitudes, the practices about HCV among laboratory technicians, and nursing staff are matters of concern. Educational programs should be formulated to raise their awareness about HCV.