The long-term separation of industry, academia and research in most colleges and universities has hindered the establishment of the interactive digital media economic system, so there is an urgent need to find a suitable method to guide colleges and universities to carry out the innovative teaching of industry-teaching integration. This paper analyzes the existing literature, summarizes the key influencing factors of the innovative teaching of industry-academia integration in colleges and universities, and obtains a system of influencing factors of the innovative teaching of industry-academia integration in colleges and universities. The fuzzy set-DEMATEL method was used to analyze the influence degree of influence factors in the integration of university industry-teaching and innovative teaching. Five condition variables, namely, enterprise scale, discipline type, project motivation, project action, and project constraint, were selected, and single-condition necessity analysis and combined-condition sufficiency analysis were carried out by fuzzy set qualitative comparative analysis method, so as to explore the optimal path for the realization of the innovative teaching of industry-teaching integration. It is summarized that high-level entrepreneurial attitude and entrepreneurial atmosphere are strong influencing factors in innovative teaching and industry-teaching integration. The original coverage of the grouped path of large enterprises, project motivation and project constraints is 0.405123, which is the optimal path to improve the efficiency of the innovative teaching of industry-teaching integration. The work of this paper provides some references for the innovative teaching of industry-teaching integration in universities.