The cultivation of humanistic literacy of medical students is an inevitable requirement for the implementation of quality education and the cultivation of excellent medical talents, which lays the foundation for the cultivation of professional talents in society. The research constructs the evaluation index system of humanistic literacy of nursing students, adopts the hierarchical analysis method based on the G1 method to calculate the weights of the evaluation indexes of quality assessment, and combines the method of fuzzy mathematics to construct the evaluation model of humanistic literacy of nursing students. At the same time, a set of internet-based and technologically advanced humanistic literacy intelligent assessment systems was developed and tested, which are based on the dual operation of WeChat terminals and PC terminals. The results show that the intelligent assessment system is better than the traditional assessment model, and can give the evaluation results fairly and reasonably, and at the same time, through the calculation of rater reliability and content validity, the assessment model is verified to have good reliability and validity, and the values obtained are all greater than 0.9. The humanistic literacy intelligent assessment system proposed in this paper can meet the functional requirements of students’ humanistic literacy in practical application.