This paper systematizes the teaching system, teaching principles and teaching process of joint cultivation of information literacy and innovation ability. The joint cultivation is applied to practical teaching, and the results of the experiments are analyzed using comparative experiments and questionnaires to analyze the changes in information literacy and innovation ability as well as the dimensions of literacy. After analyzing, it can be seen that compared with traditional teaching, the joint cultivation constructed in this study can effectively improve the information literacy and innovation ability of college students (P=0.001). Among the six dimensions, digital information innovation ability before and after the teaching practice improved by 1.057 over the average value, which is the largest. More than 50% of the students think they can use information technology equipment and tools correctly and solve practical problems creatively.