With the rapid development of education informatization, teachers’ informatization teaching ability has become a key factor to improve teaching quality. This paper constructs a training and assessment system of informatization teaching ability based on principal component analysis and logistic model. First, multiple indicators of informatization teaching ability are dimensionality reduced by principal component analysis in order to extract key principal components. Second, the main influencing factors of each dimension of informatization teaching ability are explored based on Logistic model, and the dominant degree of influencing factors on each dimension is quantified. The experimental results show that this paper’s training and assessment system of informatization teaching ability based on principal component analysis and Logistic model can effectively assess the informatization teaching ability of college language teachers and reveal the teachers’ deficiencies in the innovation of informatization teaching ability. The key influencing factors of the five important dimensions of teachers’ informatization teaching ability are also summarized to provide a scientific basis for the training and assessment of teachers’ informatization teaching ability.