This research presents a critical examination of 102 undergraduate students’ experience with e-portfolios in a university based in Northwest China. Qualitative data are collected via interviews to explore the advantages and disadvantages of e-portfolios in order to examine the possibility for popularization of the application and further improvement as well. Findings reveal that e-portfolios are beneficial in the aspects of new knowledge creation, learning management, reflective learning, test anxiety elimination, as well as student performance assessment. Meanwhile, some problems have also been revealed, which have pedagogical implications.