This paper focuses on the outcome-oriented OBE concept and the teacher information-based teaching model. Then, a regression model was constructed to study teachers’ ability to teach in information technology, and the correlation between variables was studied using regression equations and significance tests. The influencing factors of teachers’ informatization teaching ability enhancement were studied through the indicators of teacher factor, school factor, and policy training, and the strategies of teachers’ informatization teaching ability enhancement in higher education institutions were proposed. The study showed that the overall mean value of teachers’ factors among the influencing factors was 4.09, and the mean values of motivation and self-efficacy were greater than 4 points. The overall mean value of school factors was 3.9, the overall mean value of policy training was 3.55, and the mean values of training, policy, and competition were all greater than 3. This study provides some references and ideas for improving teachers’ information technology teaching abilities in higher education institutions.