In this paper, the fingerprints of 10 batches of Angelica sinensis herbs and 5 batches of commercially available Angelica sinensis preparations originating from different parts of China were determined by high-performance liquid chromatography (HPLC) fingerprinting using a methanol-acetonitrile solution system with linear gradient elution, and the similarity analyses were applied for the classification. Derringer’s satisfaction function evaluated the acceptable limit values for processing multiple characteristic peaks of Angelica sinensis herbs. The results showed that under selected chromatographic conditions, HPLC fingerprints of Angelica sinensis were obtained, and four characteristic peaks were calibrated. The similarities of 10 batches of Angelica sinensis herbs ranged from 0.953 to 0.998, and those of 5 batches of Angelica sinensis preparations ranged from 0.969 to 0.999. The method established in this paper is stable, reliable and reproducible, and it can be used to provide a reference for quality control of the herbs of Angelica sinensis and the drinkable tablets of Angelica sinensis body and to provide a reference for quality control of the classic prescriptions containing Angelica sinensis or the It can provide a reference for the quality control of Angelica sinensis herb and Angelica sinensis body tablets, and provide a basis for the development and research of classical prescriptions containing Angelica sinensis or Angelica sinensis.