Strengthening the management of science and technology resources and improving the intensive management of science and technology resources is a shortcut for the development of traditional industries to informatization. This paper analyzes a series of problems in human resource allocation by studying the current situation of enterprise informationization project implementation and constructs a human resource management system in informationization project implementation by combining relevant theoretical systems. The system uses the key chain method to find capacity-constrained resources and takes resource balancing measures, which can solve the multi-project resource allocation problem where the types and quantities of available resources have been determined. To ensure the convergence and uniform distribution of the resource allocation solution space, an orthogonal design method is used for the initialized population, and the solution space is expanded without significant changes in production costs and benefits compared to the original reference solution. After the enterprise used the system improvement, in the enterprise recruitment sales class, technical class, and administrative class onboarding rate increased by 13.2%, 57.3%, and 19.7%, respectively, the overall onboarding rate increased by 46.9%, the enterprise overall staff turnover rate reduced from 47.2% to 27.3%, technical class employees both time incentive ratio exceeded 60%. It can be seen from the information technology project that technical talent introduction, as well as technical team building, strengthening personnel training efforts, and establishing a reasonable incentive assessment system, can effectively improve the project team’s human resources management level.