As a convenient and easily accessible green space place in the environment, the countryside provides people with the opportunity to get in touch with nature intimately, so in the process of this tourism experience, people’s health benefits have been more and more widely concerned by academia. Based on this, this study utilizes the questionnaire survey method and scientific experiments to conduct an in-depth exploration of the mechanism of the impact of rural tourism activities on the physical and mental health of tourists by taking tourists who have had rural tourism experiences as the research object. This paper derives the physical and mental health indicators of tourists before tourism based on the evaluation method of physiological indicators and the test method in psychology, proposes a perceptual measurement tool to study the obtained data of tourists’ state of mind, and concludes that after tourism, the overall mean values of tourists’ average heart rate, minimum heart rate, and maximum heart rate were reduced by 0.65 bpm, 3.28 bpm, and 7.5 bpm, respectively. The mean value of ventilation per minute was reduced by 1.11 L- min-1, indicating that there is a significant effect of different ages on the physical and mental health benefits of tourists. Tourists with tour time under half an hour had a poor state of mind, and tour time of 4 hours or more had the highest POMS index of 298, with a more favorable emotional state, indicating that there is also a significant effect of tour time length on tourists’ state of mind.