This study systematically outlines the connotation and theoretical basis of project-based learning, integrates the theory of project-based learning into the teaching of business English courses in colleges and universities, and deliberately designs a business English teaching mode based on project-based learning. Starting from the purpose of the study, the research problem is determined in the hypothesis, the research subjects of this paper are selected, the experimental group and the control group are divided, and the comprehensive questionnaire, experimental process and independent sample t-test are used to verify the practical effect of the teaching mode of this paper. The empirical analysis shows that there are significant differences between this teaching mode and the traditional teaching mode in the three aspects of students’ learning interest, performance and expression ability, which satisfy the condition of P<0.05 significant difference, confirming the effectiveness of the business English teaching mode based on project-based learning.