In the multimedia era, the application of digital technology in physical education and training is becoming increasingly important. This paper analyzes how digital technology can improve biological education teaching effectiveness and students’ motivation and independent learning ability. The research methodology uses digital physical education teaching and curriculum integration, motion retrieval technology, and motion editing and scoring technology. It is found that the physical education teaching mode using digital technology is significantly better than the traditional method, in which the advantage of the class using digital technology in the mastery of physical education skills is obvious. The Analysis of final grades shows that the number of experimental courses with more than 90 points is three times as many as that of the control class. The average grade, learning motivation, and independent learning ability are higher than in traditional teaching. The specific data show that the mean value of understanding motivation in the experimental class is 26.47, and that in the control class is 24.87 (P=0.0031), which shows that the digital technology significantly improves students’ motivation to learn. The application of digital technology in physical education can dramatically improve the teaching effect, enhance students’ learning motivation and independent learning ability, an important development direction of modern physical education.