Aesthetic rules are the basis of animation art design, combined with the user-centered design method is a new form of multimedia animation art design. This paper combines the existing three-dimensional animation art forms of expression, the use of points, lines, and surfaces to generate three-dimensional animation three-dimensional space, and selected virtual reality technology to achieve three-dimensional animation conversion. Based on ray tracing algorithm and radiance global illumination technology, to optimize animation scene realism, analyze the difficulties of rendering image styles. Combined with the Canny edge extraction algorithm to extract the target area contour, homogenization processing of local area coloring, fusion of the extracted contour and coloring area, the formation of animation style rendering, constituting the design of this paper three-dimensional animation art design expression. Analyze the rendering data of trees in a typical natural scene and use design evaluation indexes to assess the performance impact of animation scene art design. The average score for the animation scene expressing information content, the average score for the animation matching with the information, and the average score for the animation effect art and beauty are 7.672, 7.027, and 7.474, respectively. The scores indicate that the animation art design expression proposed in this paper can provide a more accurate depiction of the aesthetics of animation art.