The Nile River quality being the problem concerned with everyone's health in Egypt. The Nile River is currently facing tremendous pressure due to encroachments, discharge of untreated domestic and industrial waste, dumping of solid waste and illegal diversion of water. The present study aimed to examine the bacterial quality of River Nile water at Assiut region. The current work was done in nine sampling locations distributed over about 120 km stretch at Assiut Governorate around the Nile River, for monitoring the microbial pollution by determination of total bacterial counts as well as bacterial indicators (total Coliform, fecal Coliform, and fecal Streptococci) for river Nile water. Moreover study of some physicochemical parameters during the period from September 2013 to August 2014, in order to evaluate its suitability for the intended purpose. The results showed that all of samples were contaminated with (total Coliforms, fecal Coliforms and fecal streptococci). The physicochemical parameters of the water samples of the most studied locations were in permissible limits of the Egyptian standards for drinking water. The present study observed that the overall water quality of the Nile River in the study area was ranged from marginal to good according to Egyptian laws of the Nile River protection agency.