The present study is an attempt to evaluate the groundwater for drinking and domestic uses in Diruot district through chemical and biological analysis of water samples using a Geographic Information System (GIS) technique. The study area is bounded by latitudes 27° 25 ' and 27° 40' N and longitudes 30° 45' and 30 ° 55 ' E. The goal of the study was verified by the acquisition of the available surface and subsurface geological data, collection of groundwater samples of about 27 wells distributed all over the area in different depths and carrying out the appropriate chemical and bacteriological analysis of these samples. The chemical results for well samples at depth less than 60 m were found principally acceptable for drinking use according to the Egyptian standards. According to those standards, statistically, some well samples at depths less than 60 m were not considered potable water. Also The shallow wells ( less than 50 m depth) are affected by bacteriological pollution from sewage water wells and the pollution degree are most increased near to the river Nile and Al-Ibrahimiyyah canal because there is a direct contact with these wells.