Urinary tract infection (UTI) is the second most common type of infections in the body and it affects millions of people each year. The increasing rate of antimicrobial agents' consumption to treat infections has resulted in emergence of resistance even to more potent antibiotics. A total of 12 bacterial clinical isolates were collected from chronic hemodialysis (one isolate) and out-patients of UTI (11 isolates) of International hospital for Urology and Nephrology, El-Giza, Egypt, during 7/2013:12/2013. The clinical isolates have been investigated against 20 different antibiotics. Eleven of the clinical isolates out of 12 showed resistance against all tested antibiotics and the other isolate showed resistance against 19 tested antibiotics. The isolates were resistant to imipenem (IMP), amikacin (AK), cefepime (FEP) and tigecycline (TIG) antibiotics alone or in combinations. The combination (AK/IMP/TIG/FEP) was the only one which achieved > 90% killing against all of the isolates. The three combinations (AK/TIG/IMP, TIG/IMP/FEP and TIG/AK/FEP) also achieved > 90% killing against the isolate Staphylococcus sp. (1F).