The aim of this study was to determine fungal isolates on seed samples of wheat, barley, bean, corn, faba bean, melon, chickpea and leek collected from the fields located on Van region, and their pathogenicity. Generally, it was found that seed samples were highly contaminated with Penicillium spp., Fusarium spp. and Alternaria spp. Especially Fusarium graminearum was highly isolated from wheat and barley, Fusarium oxysporum was highly obtained from melon, leek and corn seeds. Macrophomina phaseolina was isolated intensely from bean and faba bean seeds. Pathogenicity test of species were evaluated on seeds under green house conditions. Disease severity, root length, fresh and dry weights were evaluated 8-10 weeks after sowing. The experiment was conducted two times in four replications. As a result of pathogenicity tests, F. graminearum, F. oxysporum and M. phaseolina isolates caused disease severity between 68,6 % and 72 % on wheat and barley, 83 %, 43 % and 65,3 % on melon, leek and corn, 75,2 % and 68,4 % on bean and faba bean seeds, respectively. Other fungi have caused less pathogenicity.