Purpose: The purpose of this research is to examine protective effects of higenamine on testis injured by the ischemia reperfusion. Material and Method: In our experiment, the rats were separated as 3 groups including sham control, ischemia reperfusion, and ischemia reperfusion+higenamine. Some oxidant, antioxidant and inflammatory parameters were evaluated in testis tissues obtained at the end of the experiment. Findings: In current study, we observed that the oxidant and inflammatory parameters increased and antioxidant parameters decreased in the ischemia reperfusion group but the antioxidant parameters increased and oxidant parameters decreased in treatment group suggesting that single dose administration of higenamine is protective against testicular oxidative damage resulted from ischemia reperfusion.