The purpose of this research is to examine the protective effects of barbaloin on testes injury induced by ischemia reperfusion. In the experimental stage of our research, the rats were assigned to 4 groups. Our groups are scheduled as follows; sham, ischemia reperfusion, ischemia and reperfusion + DMSO, and ischemia reperfusion + barbaloin. Some oxidant and antioxidant parameters were evaluated in testes tissues obtained at the end of the experiment. In the ischemia reperfusion group, it was found that the oxidant parameters increased and antioxidant parameters decreased; on the contrary, the antioxidant parameters increased and oxidant parameters decreased in the treatment group. These results demonstrated that barbaloin administration performed a protective effect against oxidative testes injury induced by ischemia reperfusion.