Hair dyes are immensely popular in modern society. However, impact of hair dye and its residual discharged to the environment in relation to human health and ecological imbalance have not been widely studied. The current study demonstrated the cytotoxicity and genotoxcity effect on aquatic microbes and Allium cepa. Cytotoxicity of dye was significantly noted on aquatic microbes. The subdued cell division, chromosome abnormality and lower mitotic index in A. cepa reflect the presence of cytotoxic and genotoxic compounds in dye. Spectroscopy studied exhibited positive DNA interaction with dye. Aquatic system can accumulate pollutants directly or indirectly from water contaminated with cosmetic effluents from domestic and hair spa.