This study was conducted to identify the species of Pseudomonas present in the Imus River Watershed and determine the antibiotic resistance and metal tolerance patterns of Pseudomonas isolates. Water samples were collected from five stations, namely: Balite Bridge (Station 1), Dasmariñas Bridge (Station 2), Palanan Bridge (Station 3), Imus Toll Bridge (Station 4) and Island Cove Bridge (Station 5). The highest total plate count and Pseudomonas count was obtained in Dasmariñas Bridge Station (1.40 x 10⁸ and 9.65 x 10⁷), followed by Palanan Bridge Station (1.92 x 10⁵ and 9.7 x 10³), Island Cove Balite Bridge Station (1.64 x 10⁴ and 4.4 x 10²), Bridge Station (1 x 10⁴ and 3.4 x 10²) and Imus Toll Bridge Station (5.2 x 10³ and 4.01 x 10³). The Pseudomonas isolates were identified as P. aeruginosa and P. stutzeri. Antibiotic susceptibility testing revealed that all isolates were found resistant to ampicillin, amoxicillin, cephalexin and erythromycin, and susceptible to streptomycin, ofloxacin, doxycycline and tetracycline. All P. stutzeri showed resistance to clindamycin compared to 77.89% of P. aeruginosa isolates. Twenty-one (22.11%) and 74 (77.89%) isolates of P. aeruginosa exhibited tetra- and penta-drug resistance, respectively while P. stutzeri isolates exhibited penta-drug resistance. Majority of Pseudomonas isolates were resistant to 400 ppm concentration of heavy metals with exception of mercury and cobalt. They were found tolerant to 800 ppm concentration of zinc and arsenic. Penta- and hexa-tolerance of isolates were observed at 400 ppm concentration of heavy metals while bi- and tri-tolerance were recorded on 800 ppm concentration of heavy metals.