With the research of urban historical landscape, its definition has been continuously deepened. The development of a city is regarded as the result of the accumulation of cultural and natural values in different periods, that is, the historical landscape of a city is the result of historical ‘layering.’ This paper takes the historical dynamic layering process of the Largo and the architectural landscape of Macau as the research content and uses the layering method to take the economy, society and culture of Macau in each historical period as the internal cause of its layering value, relating its explicit characteristics so as to explore the ways and rules of its historical layering. Moreover, in the historical layering of various dimensions, the harmonious Chinese and Western cultural values of Macau are always linked, and it is concluded that ‘the characteristics of pluralism and symbiosis have created the present Macau.’ This paper advocates that in the protection and inheritance of the urban context of Macau, we should focus on the effective protection and promotion of the multi-symbiotic culture and pass on the unique flavour of the city jointly created by the Chinese and Portuguese people.