The article borrows game theory to analyze the development dilemma of new rural cooperative economic organizations. A rural cooperative economic organization that is based on game theory is proposed by describing the new rural cooperative economic organization and game theory. According to the subject of interest, the basic assumptions of the model are determined, and the game theory model for rural cooperative economic organizations is constructed by combining the basic assumptions and relevant variables, and the evolutionary stabilization strategy solution process is given at the same time. The development status of new rural cooperative economic organizations in the context of information technology is evaluated using the model mentioned above. When E2 = 0.18, the probability of both parties choosing {cooperation, cooperative} strategy will increase to 82.3%. And E2 = 0.82 will reduce the probability of forming complete cooperation to 71.5% through the introduction of cost and benefit distribution, supervision and punishment mechanisms to achieve a win-win situation for the community of interest, but there is still the probability of the two sides cooperating to choose the optimal strategy will become smaller, revealing that the current development of the rural cooperative economic organization has problems and shortcomings. In order to improve the development of cooperative economic organizations in rural areas, relevant strategies are proposed.